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B3428 Sigma

Anti-Bax antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins, Bioactives, Markers, and Receptors for Stem Cell Biology, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Apoptosis,
species reactivity   rat, mouse, human
application(s)   microarray: suitable
  western blot: 1:2,000 using whole cell extract of MCF7 human breast adenocarcinoma cell line
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 23 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BAX(581)
mouse ... Bax(12028)
rat ... Bax(24887)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide GGPTSSEQIMKTGALLLQGF-K corres- ponding to the N-terminal region of Bax of human origin (Bax α, amino acids 11-30 with C-terminally added lysine), conjugated to KLH as immunogen. This sequence is identical in human Bax β and highly conserved in rat and mouse Bax α (single amino acid substitution) and human Bax.

General description

Bax is a 21kD integral organelle membrane protein that belongs to the Bcl-2 family and is expressed mainly in the mitochondria. It exist in three cytosolic forms Bax β (24 kDa), Bax γ (5 kDa) and Bax δ (16 kDa) generated by alternative splicing.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-BMPR2 (836-850) antibody produced in rabbit is suitable for western blotting at a concentration of 1:500-1:2,000.

Biochem/physiol Actions

BAX is a proapoptotic protein that forms homodimer to accelerate apoptotic death whereas its heterodimerization with Bcl-2 or Bcl-xL can block apoptosis. BAX binds to the mitochondrial permeability transition pore complex (PTPC), and when expressed in cells, it induces mitochondrial dysfunction and the release of cytochrome C, leading to activation of caspases and triggers apoptosis. Loss-of-function mutation in the BAX gene results in Hematopoietic malignancies.

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