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B3782 Sigma

Restriction Enzyme Buffer SL

10 ×

Synonym: Restriction Endonuclease Buffer

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Properties

Related Categories Buffers for Restriction Enzymes, Molecular Biology, Molecular Biology Enzymes More...
description   10× buffer contains 100 mM Tris-HCl, pH 7.5 at 37°C, 100 mM MgCl2, 10 mM DTE
InChI Key   YNZJHLFHFNCZIE-UHFFFAOYSA-N
concentration   10 ×
storage temp.   −20°C

Description

General description

This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Restriction Enzyme Buffer Reference

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer ...
Keywords: Buffers, Digestions, Enzyme activity, PAGE, Solid phase extractions

Restriction Enzyme Double Digest Buffer Guide

Sigma Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. When using two restriction enzymes at once, first check the enzyme activities in each bu...
Keywords: Buffers, Digestions, Solid phase extractions

Protocols

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to ...
Keywords: Asymmetric synthesis, Buffers, Cloning, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Indirect Immunofluorescense, Methylations, Molecular biology, Molecular biology techniques, Peptide synthesis, Polymorphisms, Reproduction, Separation, Sequencing

Restriction Enzyme Digest Protocol

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also...
Keywords: Buffers, Digestions, Purification

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