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B3795 Sigma

Brain-derived neurotrophic factor human

BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: Abrineurin, BDNF

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Description

Analysis Note

The bioactivity is determined by its ability to stimulate the proliferation of rat C6 cells.

Biochem/physiol Actions

Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.

Physical form

lyophilized from a sterile 0.2 μm filtered solution with BSA

Application

Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.

General description

BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Growth Factors in Stem Cell Biology

Growth factors are naturally occurring regulatory molecules, which bind to receptors on the cell surface. They stimulate cell and tissue function through influencing cell differentiation by changing ...
Keywords: Adhesion, Angiogenesis, Cell biology, Cell proliferation, Cellular processes, Diseases, Growth factors, Hormones, Stem cell biology, Therapeutic uses, Transcription, Transfection

Neurotrophic Factors

Neurotrophic factors are agents that are important for survival, growth, or differentiation of discrete neuronal populations. Based on amino acid sequence homologies of cytokines and receptors and ba...
Jennifer Fries
BioFiles 2009, 4.5, 19.
Keywords: Cell proliferation, Dopamine agents, Growth factors

Peer-Reviewed Papers
15

References

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