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B3804 Sigma

BCIP®/NBT-Blue Liquid Substrate System for Membranes

alkaline phosphatase substrate

Synonym: 5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system

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Description

Application

A ready-to-use buffered alkaline phosphatase substrate for use in immunoblotting. Produces an insoluble dark blue-purple end product. Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

Caution

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

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Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Applications, Enzyme-linked immunosorbent assay, Formulations, Immunohistochemistry, Western blot

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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