Blue-White Select™ Screening Reagent
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Properties
| Related Categories | Core Bioreagents, Featured Products, Gene Reporter Substrates, General Reagents, Life Science Reagents for Cloning, |
| usage | sufficient for 125 dishes (90 mm) |
| shipped in | dry ice |
| storage temp. | −20°C |
Description
Other Notes
A ready to use solution of 5-bromo-4-chloro-3-indoly
Application
Blue/white color selection is a routine technique that differentiates between colonies/plaques that contain cloning vector without an insert and those that contain a vector harboring the insert of interest. The technique is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an α-fragment of β−galactosidase. Exploitation of these vectors requires use of a bacteria strain carrying the complementing gene fragment to allow the assembly of an active complex. Transformation of β-galactosidase gene fragment containing vectors to the appropriate cells, in the presence of the β-galactosidase chromogenic substrate X-Gal and the inducer IPTG, results in the formation of blue colonies/plaques. Disruption of the β-galactosidase gene by insertion of a DNA fragment into the vector′s multiple cloning site results in the loss of functional β-galactosidase activity. Colonies/plaques bearing an insert containing vectors will remain white and may be easily distinguished from those bearing an intact cloning vector.
Legal Information
Blue-White Select is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
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