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B4180 Sigma

Anti-BAPTA antibody produced in rabbit

1.2 mg/mL, IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium Probes,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
concentration   1.2 mg/mL
application(s)   immunoprecipitation: suitable
  indirect immunofluorescence: suitable
  western blot: suitable
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

BAPTA conjugated to KLH.

Physical form

Solution in phosphate buffered saline, pH 7.4, with 0.08% sodium azide.

Application

Anti-BAPTA antibody produced in rabbit is suitable for immunoblotting, immunofluorescence or immunoprecipitation at 1-10 μg/mL. The antibody is used in determining spatial distribution of BAPTA through immunocytochemistry and confocal microscopy of cortical neurons loaded with BAPTA/AM, an analog. It is also useful in determining kinetics of loading and efflux of BAPTA through ELISAs and immunocytochemistry.

General description

BAPTA is a highly selective calcium chelator. BAPTA and its analog BAPTA/AM have been extensively used in cell physiology research for defining the mechanisms underlying inter- or intracellular calcium homeostasis and calcium dependent exocytosis. They may also be used in the study of diseases in which the regulation of excitatory neurotransmitter release and uptake has been disturbed.

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