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B4186 Sigma

Butyrylcholinesterase from human serum

vial of ≥4 units

Synonym: Acylcholine acyl-hydrolase, Choline esterase, butyryl, Pseudocholinesterase

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Starting material individually donor tested and found negative for HIV I & II antibodies, Hepatitis B surfaceantigen, and Hepatitis C antibodies.

Unit Definition

One unit will hydrolyze 1.0 μmole of butyrylcholine to choline and butyrate per min at pH 8.0 at 37 °C. The activity obtained using butyrylcholine as substrate is ~2.5 times that obtained using acetylcholine.

Application

Butyrylcholinesterase (BChE) may be used to hydrolyze large esters of choline such as butyrylcholine and benzoylcholine. Selective inhibition of BChE activity can be used in the detection of organophosphates. BchE may be used to treat organophosphate toxicity. It may be a useful tool in Alzheimer’s research. BchE, from Sigma, has been used to determine butyrylcholinesterase inhibition by various test compounds .

Biochem/physiol Actions

Butyrylcholinesterase (BChE) is a serine hydrolase that shares substantial structural similarities with acetylcholinesterase (AchE) but has different substrate and inhibitor specificities. BChE is found in the serum, hemopoietic cells, liver, lung, heart and the central nervous system of vertebrates.

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All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What amount of enzyme is present in 1 vial of Product B4186, Butyrylcholinesterase from human serum?
Each vial will contain at least 5 units. The vial content is given on the certificate of analysis of each lot. The vial may be substantially overpacked.
How should Product B4186, Butyrylcholinesterase from human serum, be reconstituted?
The enzyme can be reconstituted in 20 mM sodium phosphate buffer, pH 7.0, with 0.15 M sodium chloride.
Are any buffer salts present in Product B4186, Butyrylcholinesterase from human serum?
This product was lyophilized from 20 mM ammonium bicarbonate, which is a volatile buffer. So it would be expected to contain only traces of buffer salts.
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Protocols & Articles

Articles

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

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Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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