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  • B4500 - Nα-Benzoyl-L-arginine ethyl ester hydrochloride


B4500 Sigma

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate

Synonym: BAEE




The prototype substrate for trypsin.

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Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Enzymatic Assay of Trypsin Inhibitor

2. SCOPE This procedure applies to all products that have a specification for Trypsin Inhibition at Sigma-Aldrich, St. Louis.

Enzymatic Assay of Trypsinogen

1. OBJECTIVE To standardize a procedure for the determination of the enzymatic assay of trypsinogen.
Keywords: Enzyme activity, Extinction coefficient

Procedure for Enzymatic Assay of Trypsin (EC

This procedure is for products with a specification for Trypsin activity using Nα-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determina...

Peer-Reviewed Papers


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Immobilization of papain on cold-plasma functionalized polyethylene and glass surfaces. Ganapathy R, Manolache S, Sarmadi M, et al. J. Biomater. Sci. Polym. Ed. 12(9), 1027-49, (2001)


Effect of PEG molecular weight and linking chemistry on the biological activity and thermal stability of PEGylated trypsin. Treetharnmathurot B, Ovartlarnporn C, Wungsintaweekul J, et al. Int. J. Pharm. 357(1-2), 252-9, (2008)


[Determination of biological activity of extract from hirudo by N-benzoyl-L-arginine ethyl ester]. Guan S, Peng C, Li H, et al. Zhongguo Zhong Yao Za Zhi 35(15), 1990-2, (2010)


Micropit: A New Cell Culturing Approach for Characterization of Solitary Astrocytes and Small Networks of these Glial Cells. Lee W, Malarkey EB, Reyes RC, et al. Front. Neuroeng. 1, 2, (2008)


[Effect of trypsin microenvironment on the rate constants of elementary stages of Nalpha-benzoyl-L-arginine ethyl ester hydrolysis]. Zakharchenko NL, Ermakova EA, and Zuev IuF Bioorg. Khim. 34(3), 404-8, (2008)


Enhanced proteolytic activity of covalently bound enzymes in photopolymerized sol gel. Dulay MT, Baca QJ, and Zare RN Anal. Chem. 77(14), 4604-10, (2005)


Enzyme-friendly, mass spectrometry-compatible surfactant for in-solution enzymatic digestion of proteins. Yu YQ, Gilar M, Lee PJ, et al. Anal. Chem. 75(21), 6023-8, (2003)


Kinetic characterization of protein arginine deiminase 4: a transcriptional corepressor implicated in the onset and progression of rheumatoid arthritis. Kearney PL, Bhatia M, Jones NG, et al. Biochemistry 44(31), 10570-82, (2005)


Recent developments in methods and technology for analysis of biological samples by MALDI-TOF-MS. Pan C, Xu S, Zhou H, et al. Anal. Bioanal. Chem 387(1), 193-204, (2007)


Anti-PAD4 autoantibodies in rheumatoid arthritis: levels in serum over time and impact on PAD4 activity as measured with a small synthetic substrate. Pollmann S, Stensland M, Halvorsen EH, et al. Rheumatol. Int. 32(5), 1271-6, (2012)


Sodium-deoxycholate-assisted tryptic digestion and identification of proteolytically resistant proteins. Lin Y, Zhou J, Bi D, et al. Anal. Biochem. 377(2), 259-66, (2008)


Cyanopeptoline CB071: a cyclic depsipeptide isolated from the freshwater cyanobacterium Aphanocapsa sp. Choi, H., et al. Chem. Pharm. Bull. 56, 1191-3, (2008)


Kinetic and structural properties of two isoforms of trypsin isolated from the viscera of Japanese anchovy, Engraulis japonicus. Ahsan MN and Watabe S J. Protein Chem. 20(1), 49-58, (2001)


Protein arginine deiminase 4: evidence for a reverse protonation mechanism. Knuckley B, Bhatia M, and Thompson PR Biochemistry 46(22), 6578-87, (2007)


Transglutaminase-catalyzed synthesis of trypsin-cyclodextrin conjugates: kinetics and stability properties. Villalonga R, Fernández M, Fragoso A, et al. Biotechnol. Bioeng. 81(6), 732-7, (2003)


A vast amount of enzyme activity fails to be absorbed within the human pancreas: implications for cost-effective islet isolation procedures. Friberg AS, Korsgren O, and Hellgren M Transplantation 95(6), e36-8, (2013)


Immobilization of trypsin on sub-micron skeletal polymer monolith. Yao C, Qi L, Hu W, et al. Anal. Chim. Acta 692(1-2), 131-7, (2011)


Trypsin from the viscera of Bogue (Boops boops): isolation and characterisation. Barkia A, Bougatef A, Nasri R, et al. Fish Physiol. Biochem. 36(4), 893-902, (2010)


Effects of nitric oxide donors and nitric oxide synthase substrates on ciliary muscle contracted by carbachol and endothelin for possible use in myopia prevention. Beauregard C, Liu Q, and Chiou GC J. Ocul. Pharmacol. Ther. 17(1), 1-9, (2001)


Quantification of leonurine, a novel potential cardiovascular agent, in rat plasma by liquid chromatography-tandem mass spectrometry and its application to pharmacokinetic study in rats. Zhu Q, Cai W, Sha X, et al. Biomed. Chromatogr. 26(4), 518-23, (2012)


Development of broad-spectrum halomethyl ketone inhibitors against coronavirus main protease 3CL(pro). Bacha U Chem. Biol. Drug Des. 72(1), 34-49, (2008)


Role of guanidyl moiety in the insertion of arginine and Nalpha-benzoyl-L-argininate ethyl ester chloride in lipid membranes. Fonseca AC, Frías MA, Bouchet AM, et al. J. Phys. Chem. B 114(17), 5946-52, (2010)


Critical assessment of the spectroscopic activity assay for monitoring trypsin activity in organic-aqueous solvent. Crowell AM, Stewart EJ, Take ZS, et al. Anal. Biochem. 435(2), 131-6, (2013)


Effect of proteolytic activity of Epicoccum purpurascens major allergen, Epi p 1 in allergic inflammation. Kukreja N Clin. Exp. Immunol. 154(2), 162-71, (2008)


A trypsin homolog in amphioxus: expression, enzymatic activity and evolution. Feng W and Zhang S Mol. Biol. Rep. 39(2), 1745-53, (2012)


Brownian dynamics simulation of substrate motion near active site of enzyme entrapped inside reverse micelle. Ermakova EA, Zakhartchenko NL, and Zuev YF Eur. Biophys. J. 39(9), 1335-41, (2010)


Assessment of the enzymatic activity and inhibition using HPFA with a microreactor, trypsin, absorbed on immobilized artificial membrane. Liu Y, Li L, Dai R, et al. J. Chromatogr. Sci. 48(2), 150-5, (2010)


Protective effect of Carbopol on enzymatic degradation of a peptide-like substrate. I: Effect of various concentrations and grades of Carbopol and other reaction variables on trypsin activity. Vaidya AP, Wigent RJ, Moore JC, et al. Pharm. Dev. Technol. 12(1), 89-96, (2007)


Preparation and characterization of a truncated caricain lacking 41 residues from the N-terminal. Liu W, Ye W, Wang Z, et al. Protein J. 24(4), 243-51, (2005)


Beil. 9,III,1232

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