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B5161 Sigma

Biotin 3-sulfo-N-hydroxysuccinimide ester sodium salt

≥90% (TLC), powder

Synonym: Sulfosuccinimidyl biotin

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Properties

Related Categories Biotinylation, Molecular Biology, Protein Modification, Protein Structural Analysis, Proteomics,
InChI Key   NKHCQKKDLWGBRK-UHFFFAOYSA-N
assay   ≥90% (TLC)
form   powder
solubility   H2O: soluble10 mg/mL
  DMF: soluble50 mg/mL
storage temp.   −20°C

Description

Application

Water soluble biotinylation reagent. Typically coupled to primary amine in the pH range 6.5-8.5.

Packaging

10, 50 mg in glass bottle

General description

Biotin:avidin second antibody signal systems involve the conjugation of biotin to a secondary antibody. Once the secondary antibody binds to a target primary antibody in an immunochemical assay, the signal is developed by allowing avidin which has been conjugated to an enzyme or signal molecule to bind to the antibody associated biotin molecule.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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