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B5525 Sigma

Bromophenol Blue sodium salt

Synonym: 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt




A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Bromophenol blue sodium salt is used as tracking dye in electrophoresis. It was used in the preparation of protein samples for western blotting analysis.1 The dye was used in Drosphila fly food to aid visualization of gut for dissection.2

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.


5, 10, 25 g in glass bottle


A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Price and Availability


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Bromophenol Blue

ACS reagent

Bromophenol Blue sodium salt

for molecular biology, for electrophoresis

Bromophenol Blue sodium salt

Dye content 90 %, ACS reagent

Bromophenol Blue solution

0.04 wt. % in H2O

Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Peer-Reviewed Papers


Set your institution to view full text papers.

1. Suppression of fever at near term is associated with reduced COX-2 protein expression in rat hypothalamus. Mouihate A, Clerget-Froidevaux MS, Nakamura K, et al. Am. J. Physiol. Regul. Integr. Comp. Physiol. 283(3), R800-5, (2002)


2. Why flies? Inexpensive public engagement exercises to explain the value of basic biomedical research on Drosophila melanogaster. Pulver SR, Cognigni P, Denholm B, et al. Adv. Physiol. Educ. 35(4), 384-92, (2011)


Multidimensional proteomic analysis of proteolytic pathways involved in cell cycle control. Schmidt MW, Jain A, and Wolf DA Methods Mol. Biol. 241, 235-45, (2004)


Modified murine intracranial aneurysm model: aneurysm formation and rupture by elastase and hypertension. Hosaka K, Downes DP, Nowicki KW, et al. J. Neurointerv. Surg., doi:10.1136/neurintsurg-2013-010788, (2013)

Merck 14,1444

Beil. 19,I,649

FT-IR2 2 (3), 4143:B / RegBook 1 (2), 2789:E / Sigma FT-IR 1 (2), 111:C / Structure Index 1, 443:B:7

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