B5525 Sigma

Bromophenol Blue sodium salt

Synonym: 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt



A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Bromophenol blue sodium salt is used as tracking dye in electrophoresis. It was used in the preparation of protein samples for western blotting analysis.1 The dye was used in Drosphila fly food to aid visualization of gut for dissection.2

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.


5, 10, 25 g in glass bottle


A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

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