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B6024 Sigma

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

tablet

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

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Properties

Related Categories Chromogenic, Galactoside, Gene Reporter Substrates, Glycobiology, Inhibitors and Substrates,
InChI Key   OPIFSICVWOWJMJ-AEOCFKNESA-N
form   tablet
suitability   suitable for identification of lac+ bacterial colonies
storage temp.   −20°C

Description

Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Quantity

Contains 5 mg substrate per tablet

Reconstitution

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

Peer-Reviewed Papers
15

References

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