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B6061 Sigma

Anti-Beclin 1 (N-terminal) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-ATG6, Anti-BECN1, Anti-Coiled-coil myosin-like BCL2-interacting protein, Anti-GT197, Anti-VPS30

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Autophagy, Antibodies for Cell Biology, Antibodies for Detecting Autophagy,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100 using biotin/ExtrAvidin®-Peroxidase staining of heat-retrieved sections of human cerebellum
  immunoprecipitation: 1-2 μL using extract of human HEK-293T cells expressing recombinant human Beclin 1 fusion protein
  indirect immunofluorescence: suitable
  western blot: 1:250-1:500 using whole extract of human HeLa cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt ~60 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BECN1(8678)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 29-46 of human Beclin 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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B6186 Anti-Beclin 1 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

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