• USA Home
  • B6185 - Bionic Buffer, 10X Concentrate

EMAIL THIS PAGE TO A FRIEND
B6185 Sigma

Bionic Buffer, 10X Concentrate

for Northern and Southern blotting, solution

Synonym: Sodium Borate Electrophoresis Buffer

Purchase

Properties

Related Categories Buffers / Buffer salts, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Research Essentials
grade   for molecular biology
form   solution
impurities   DNase, RNase and nickase, none detected
storage temp.   room temp

Description

Caution

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Features and Benefits

• Faster run times
• Sharper bands
• Easy to dilute and use
• Flexible use with a variety of gel types

General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Packaging

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Application

Suitable for:
• Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
• Use with TBA and TAE gels
• Preparation of agarose gels

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Articles

Bionic™ Buffer FAQs

From our library of Articles, Sigma-Aldrich presents Bionic™ Buffer FAQs
Keywords: Cloning, Electrophoresis, Polymerase chain reaction, Purification

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Related Content

Bionic™ Buffer Data

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for:
Keywords: Buffers, Electrophoresis, Polymerase chain reaction, Separation

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

B6429 Casein Blocking Buffer 10x, for Northern and Southern blotting, solution
D0428 1 kb DNA Ladder, for DNA electrophoresis
H7033 PerfectHyb Plus Hybridization Buffer, for Northern and Southern blotting, solution
T9285 Tris-EDTA buffer solution, 100X concentrate for Northern and Southern blotting
S6639 SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution
GERPN3005 ECL Direct Nucleic Acid, GE Healthcare, RPN3005, pack of 1 ea
D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?