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B6186 Sigma

Anti-Beclin 1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-ATG6, Anti-BECN1, Anti-Coiled-coil myosin-like BCL2-interacting protein, Anti-GT197, Anti-VPS30

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Autophagy, Antibodies for Cell Biology, Antibodies for Detecting Autophagy,
species reactivity   human, mouse, rat
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5-10 μg/mL using biotin/ExtrAvidin®-Peroxidase staining of heat-retrieved sections of rat brain
  immunoprecipitation: 1-2 μL using extract of human HeLa cells
  indirect immunofluorescence: suitable
  western blot: 2-4 μg/mL using whole extracts of rat PC12 and mouse 3T3 cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~60 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BECN1(8678)
mouse ... Becn1(56208)
rat ... Becn1(114558)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 329-345 of human beclin 1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

General description

Beclin 1 belongs to beclin family is vital for early embryonic development and is a haplo- insufficient tumor-suppressor gene. It plays a pivotal role in autophagy and is also crucial for abscission step of cytokinesis. Anti-Beclin 1 antibody can be used in indirect immunofluorescence. Rabbit anti-beclin 1 antibody reacts specifically with beclin 1 of human, rat and mouse.

Beclin 1, a Bcl2 interacting protein, is the mammalian ortholog of yeast Atg6/Vps30. Beclin 1 is able to complement the autophagy deficiency of yeast lacking Atg6 and restores autophagy in human MCF7 breast carcinoma cells. The autophagy-promoting activity of Beclin 1 in MCF7 cells is associated with inhibition of MCF7 cellular proliferation.

Beclin 1 is essential for early embryonic development and is a haplo-insufficient tumor-suppressor gene. Heterozygous Beclin 1 mutant mice have increased spontaneous tumorigenesis. Beclin 1 is monoallelically deleted in a high percentage of sporadic human breast, ovarian and prostate carcinomas, and is expressed at reduced levels in those tumors. Beclin 1 decreases in an age dependent fashion in human brains, possibly leading to a reduction of autophagic activity during aging, which may contribute to the accumulation of mutant Huntingtin and the age-delayed onset of Huntington disease.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Anti-Beclin 1 recognizes human, rat, and mouse Beclin 1 by immunoblotting (~60 kDa), immunoprecipitation, and immunohistochemistry. Detection of the Beclin 1 band by immunoblotting is specifically inhibited by the immunizing peptide.

Application

Anti-Beclin 1 antibody can be used in western blotting and immunohistochemistry.

Anti-Beclin 1 antibody may be used for Immunoblotting: a working concentration of 2-4 mg/mL is recommended using whole extracts of rat PC12 or mouse 3T3 cells and Immunoprecipitation: A working amount of 1-2 mL is recommended using extracts of human HEK-293T cells expressing recombinant human Beclin 1 fusion protein.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Co. LLC

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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