BCIP®/NBT solution, premixed

Properties

Related Categories

Alkaline Phosphatase, Biochemicals and Reagents, Colorimetric, Core Bioreagents, Detection,

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Description

Other Notes

0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl₂, pH approx. 9.2

Substrates

Substrate for visualizing alkaline phosphatase conjugates in Western blotting.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

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Documents

Certificate of Analysis

B6404 - Bulletin (79 KB)

Safety Information

Personal Protective Equipment

Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)

Technical information & documentation associated with this product is available in the Safety & Documentation tab.

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

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BCIP^®/NBT solution, premixed