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B6429 Sigma

Casein Blocking Buffer 10x

for Northern and Southern blotting, solution

Purchase

Description

Features and Benefits

• Better blocking performance than dried milk or BSA
• Easy-to-use concentrate
• Compatible with neutral and positively-charged membranes
• Compatible with chemiluminescent and colorimetric detection assays

General description

Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.

Preparation Note

Dilute 10x Blocking Buffer with molecular biology grade water before use.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Protocols

10x Blocking Buffer

Product Description Precautions and Disclaimer Storage/Stability Reagents Required but Not Provided Preparation Instructions Procedure Related Products Materials References
Keywords: Buffers, Nucleic acid hybridization

Peer-Reviewed Papers
15

References

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