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B6650 Sigma

X-GlcA

reagent for selection of recombinant bacterial clones

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt, X-GlcA, X-glucuronide CHA salt

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Properties

Related Categories Chromogenic, Gene Reporter Substrates, Glucuronide, Glycobiology, Inhibitors and Substrates,
grade   Molecular Biology Reagent
  for molecular biology
InChI Key   JXCKZXHCJOVIAV-CYRSAHDMSA-N
sterility   non-sterile
assay   ≥98% (TLC)
form   powder
solubility   DMF: soluble
suitability   suitable for β-galactosidase test
storage temp.   −20°C

Description

Application

Chromogenic substrate for β-glucuronidase (GUS) gene detection.

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.

General description

X-GlcA is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. X-GlcA is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Principle

When X-GlcA is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GlcA turn an intense blue color, allowing for easy differentiation between lac+ and lac- colonies.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Fermentation: Enzymes, Substrates and SCFA

The influence of probiotics on the metabolic activity of intestinal microbiota may be evaulated by detecting bacterial enzymatic activity in fecal samples, specifically β-glucosidase, β-glucuronidase...
Chloe McClanahan
Biofiles Edition 6.2
Keywords: Absorption, Cancer, Carcinogens, Cell proliferation, Chromatography, Enzyme activity, Gas chromatography, Gastrointestinal, High performance liquid chromatography, Metabolites, Prebiotics, Probiotics, Thin layer chromatography

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

Probiotics and Human Health

At the beginning of the 20th century, probiotics were thought to have a beneficial effect to the host by improving the intestinal microbial balance. Through his studies and observations, the Russian ...
Chloe McClanahan
BioFiles Edition 6.2
Keywords: AGE, Absorption, Adhesion, Amplification, Anaerobic, Antimicrobials, Apoptosis, Cancer, Capillary electrophoresis, Carcinogens, Cell culture, Cell proliferation, Chromatography, Coagulation, Detoxification, Diagnostic, Diseases, Enzyme activity, Fermentation, Gas chromatography, Gastrointestinal, Genomics, High performance liquid chromatography, Immunology, Inflammation, Magnetic resonance spectroscopy, Metabolism, Metabolites, Microbiology, Molecular biology, Nucleic acid hybridization, Pharmaceutical, Phosphorylations, Polymerase chain reaction, Polymerase chain reaction - quantitative, Prebiotics, Probiotics, Reductions, Respiratory, Sequencing, Thin layer chromatography, Titrations

Peer-Reviewed Papers
15

References

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