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B6777 Sigma

5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt

BioReagent, for molecular biology, powder, ≥99%

Synonym: BCIP® p-toluidine salt, X-phosphate p-toluidine salt

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Properties

Related Categories BCIP/NBT Substrate Systems, Biochemicals and Reagents, Colorimetric, Detection Substrates, Enzyme Substrates,
grade   for molecular biology
InChI Key   QEIFSLUFHRCVQL-UHFFFAOYSA-N
product line   BioReagent
assay   ≥99%
form   powder
solubility   DMF: soluble20 mg/mL
  H2O: insoluble
foreign activity   Protease, none detected
storage temp.   −20°C

Description

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates

Histochemical substrate for alkaline phosphatase.

Application

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and th...
Keywords: Catalytic combustion detector, Detection methods, Northern blot, Nucleic acid hybridization, Purification, Size-exclusion chromatography, Western blot

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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