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B6804 Sigma

Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody produced in mouse

clone BAP-77, ascites fluid

Synonym: Monoclonal Anti-Phosphatase, Alkaline, bacterial

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, B-BI, Molecular Biology,
biological source   mouse
antibody form   ascites fluid
clone   BAP-77, monoclonal
mol wt   antigen mol wt ~50 kDa
species reactivity   bacteria
application(s)   indirect ELISA: suitable
  western blot: 1:20,000 using purified Escherichia coli BAP
isotype   IgG1
conjugate   alkaline phosphatase conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

A bacterial alkaline phosphatase (BAP) tagged fusion protein.

Application

Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody is suitable for use in western blot (at 1:20,000 dilutions using purified Escherichia coli BAP) and indirect ELISA.

General description

Bacterial alkaline phosphatase (BAP) is a periplasmic enzyme that is encoded by the phoA gene. This enzyme regulates the catabolism of phosphate esters. The use of a BAP tag to the target gene yields a stable fusion product that does not affect the bioactivity and biodistribution of the target gene in recombinant expression vector systems. Hence, anti-BAP antibodies can be useful tools for the detection and analysis of target biomolecules fused to BAP tags
Monoclonal Anti-BAP antibody is specific for BAP and BAP tagged proteins. The product does not detect alkaline phosphatase derived from human placenta, bovine liver, calf intestinal mucosa and shrimp. Moreover, in immunoblotting, the product does not react with endogenous BAP in whole extracts of wild type E. coli.

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Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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