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B6916 Sigma

Bradford Reagent

for 0.1-1.4 mg/ml protein

Synonym: Coomassie dye binding protein assay, Protein dye reagent

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Features and Benefits

• The reagent is ready to use. No mixing or dilution required.
• Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
• Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
• Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
• Can be used in microwell plate assays.
• Inexpensive assay.

Application

Bradford Reagent has been used to determine total protein concentration.

General description

Bradford assay is addition of coomassie brilliant blue G-250 to protein solution. The coomassie blue dye associates with basic and aromatic amino acids, thereby causing shift in absorbance during protein determination.

Price and Availability


Amplified Detection

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 1805 8 / PGIII
WGK Germany 
1

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
Will the buffer or solution my protein is in interfere with Product B6916, Bradford Reagent?
A compatibility chart is listed in the bulletin. If your substance is not listed, then we recommend testing this by diluting the standard protein samples in the same buffer as the unknown samples. To view this information on the B6916 technical bulletin, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/b6916bul.Par.0001.File.tmp/b6916bul.pdf
What is the useful concentration range that can be measured by Product B6916, Bradford Reagent?
The Bradford Reagent requires no dilution and is suitable for micro, multiwell plate, and standard (cuvet) assays. The linear concentration range is 0.1-1.4 mg/mL of protein, using BSA (bovine serum albumin) as the standard protein.  To view this information on the B6916 Technical Bulletin, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/b6916bul.Par.0001.File.tmp/b6916bul.pdf
When using Product B6916, Bradford Reagent, how soon after my color development do I need to read my assay?
he protein-dye complex is stable up to 60 minutes. The absorbency of the samples must be recorded before the 60 minute time limit and within 10 minutes of each other.  To view this information on the B6916 technical bulletin, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/b6916bul.Par.0001.File.tmp/b6916bul.pdf
When using Product B6916, Bradford Reagent, what can I do if I have a very dilute sample in a large volume?
The micro assay using this same reagent may be an option for you. The micro assay is used when a large volume (at least 1 mL) of a dilute sample is available for testing. The linear concentration range of this assay is lower than the standard or multiwell plate assays, (1-10 µg of total protein in 1 mL).  To view this information on the B6916 technical bulletin, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/b6916bul.Par.0001.File.tmp/b6916bul.pdf
Is the BSA standard supplied with Product B6916, Bradford Reagent?
No, we do not supply the BSA standard with this reagent. We recommend using one of the following products: Protein Standard (BSA) Solution, (2 mg/mL), Product No. P0834 or Protein Standard (BSA) Solution, (1 mg/mL), Product No. P0914, if lower concentrations of protein are to be measured.  To view this information on the B6916 technical bulletin, click on the following link: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Bulletin/b6916bul.Par.0001.File.tmp/b6916bul.pdf
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA™

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as ...
LSI Issue 24
Keywords: Alkylations, Amino acid analysis, Cell culture, Cell disruption, Centrifugation, Digestions, Gene expression, High performance liquid chromatography, Immobilization, Liquid chromatography mass spectrometry, Mass spectrometry, Methods, PAGE, Phase transitions, Precipitation, Proteomics, Reductions, Spectra, Transfection, Western blot

Peer-Reviewed Papers
15

References

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