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B6916 Sigma

Bradford Reagent

for 1-1,400 μg/ml protein

Synonym: Coomassie dye binding protein assay, Protein dye reagent

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Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

This protein assay is based on complexing of proteins with Brilliant Blue G. The protein sample is mixed with the reagent and then read at 595 nm after a short incubation at room temperature.

Features and Benefits

• The reagent is ready to use. No mixing or dilution required.
• Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
• Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
• Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
• Can be used in microwell plate assays.
• Inexpensive assay.

Price and Availability

Safety & Documentation
Protocols & Articles

Articles

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA™

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as ...
LSI Issue 24
Keywords: Alkylations, Amino acid analysis, Cell culture, Cell disruption, Centrifugation, Digestions, Gene expression, High performance liquid chromatography, Immobilization, Liquid chromatography mass spectrometry, Mass spectrometry, Methods, PAGE, Phase transitions, Precipitation, Proteomics, Reductions, Spectra, Transfection, Western blot

Peer-Reviewed Papers
15

References

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