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B6916 Sigma

Bradford Reagent

for 1-1,400 μg/ml protein

Synonym: Coomassie dye binding protein assay, Protein dye reagent

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Description

Frequently Asked Questions

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Application

This protein assay is based on complexing of proteins with Brilliant Blue G. The protein sample is mixed with the reagent and then read at 595 nm after a short incubation at room temperature.

Features and Benefits

• The reagent is ready to use. No mixing or dilution required.
• Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
• Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
• Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
• Can be used in microwell plate assays.
• Inexpensive assay.

Price and Availability

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
Hazard Codes (Europe) 
Xn
Risk Statements (Europe) 
Safety Statements (Europe) 
26-36/37
RIDADR 
UN 1805 8 / PGIII
WGK Germany 
1

Protocols & Articles

Articles

Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA™

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as ...
LSI Issue 24
Keywords: Alkylations, Amino acid analysis, Cell culture, Cell disruption, Centrifugation, Digestions, Gene expression, High performance liquid chromatography, Immobilization, Liquid chromatography mass spectrometry, Mass spectrometry, Methods, PAGE, Phase transitions, Precipitation, Proteomics, Reductions, Spectra, Transfection, Western blot

Peer-Reviewed Papers

References

Set your institution to view full text papers.

Why does Coomassie Brilliant Blue interact differently with different proteins? A partial answer. Tal, M., et al. J. Biol. Chem. 260, 9976, (1985)

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Bradford, M. Anal. Biochem. 72, 248-254, (1976)

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Mechanism of dye response and interference in the Bradford protein assay. Compton, S.J., and Jones, C.G. Anal. Biochem. 151, 369-374, (1985)

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Sensitivity and variability of the Bradford protein assay in the presence of detergents. Friedenauer, D., and Berlet, H.H. Anal. Biochem. 178, 263-268, (1989)

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Quantitation of microgram amounts of protein in SDS-mercaptoethanol-tris electrophoresis sample buffer. Rubin, R.W., and Warren, R.W. Anal. Biochem. 83, 773-777, (1977)

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Nonlinearity in protein assays by the Coomassie blue dye-binding method. Splittgerber, A.G., Sohl, J. Anal. Biochem. 179, 198, (1989)

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Metabolic cleavage and translocation efficiency of selected cell penetrating peptides: a comparative study with epithelial cell cultures. Foerg C AAPS J. 10(2), 349-59, (2008)

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Guggulsterone targets smokeless tobacco induced PI3K/Akt pathway in head and neck cancer cells. Muzafar A. Macha et al PLoS ONE 6, e14728, (2011)

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The endogenous brain constituent N-arachidonoyl L-serine is an activator of large conductance Ca2+-activated K+ channels. Godlewski G J. Pharmacol. Exp. Ther. 328(1), 351-61, (2009)

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Critical role for peripherally-derived interleukin-10 in mediating the thermoregulatory manifestations of fever and hypothermia in severe forms of lipopolysaccharide-induced inflammation. Harden LM, Rummel C, Laburn HP, et al. Pflugers Arch. 466(7), 1451-66, (2014)

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A cytosolic invertase is required for normal growth and cell development in the model legume, Lotus japonicus. Tracey Welham et al J. Exp. Bot. 60, 3353-65, (2009)

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Tau oligomers impair memory and induce synaptic and mitochondrial dysfunction in wild-type mice. Cristian A Lasagna-Reeves et al Molecular Neurodegeneration 6, 39, (2011)

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Transgene expression is associated with copy number and cytomegalovirus promoter methylation in transgenic pigs. Qingran Kong et al PLoS ONE 4, e6679, (2009)

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A comparison of leaf and petal senescence in wallflower reveals common and distinct patterns of gene expression and physiology. Price AM Plant Physiol. 147(4), 1898-912, (2008)

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A bovine model of respiratory Chlamydia psittaci infection: challenge dose titration. Petra Reinhold et al PLoS ONE 7, e30125, (2012)

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L-selectin ligands in human endometrium: comparison of fertile and infertile subjects. Margarit L Hum. Reprod. 24(11), 2767-77, (2009)

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Spermidine feeding decreases age-related locomotor activity loss and induces changes in lipid composition. Minois N, Rockenfeller P, Smith TK, et al. PLoS ONE 9(7), e102435, (2014)

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Parasitostatic effect of maslinic acid. II. Survival increase and immune protection in lethal Plasmodium yoelii-infected mice. Moneriz, C Malaria Journal 10, 103, (2011)

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Abnormal kinetochore-generated pulling forces from expressing a N-terminally modified Hec1. Mattiuzzo M, Vargiu G, Totta P, et al. PLoS ONE 6(1), e16307, (2011)

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Cinnamic acid, an autoinducer of its own biosynthesis, is processed via Hca enzymes in Photorhabdus luminescens. Chalabaev S Appl. Environ. Microbiol. 74(6), 1717-25, (2008)

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Effects of glutathione reductase inhibition on cellular thiol redox state and related systems Zhao, Y., et al. Arch. Biochem. Biophys. 485, 56-62, (2009)

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Expression of adipocyte biomarkers in a primary cell culture models reflects preweaning adipobiology. Chu DT, Malinowska E, Gawronska-Kozak B, et al. J. Biol. Chem. 289(26), 18478-88, (2014)

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Firefly luciferase gene contains a cryptic promoter. Vopálenský V RNA 14(9), 1720-9, (2008)

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Sodium/bicarbonate cotransporter NBCn1/slc4a7 increases cytotoxicity in magnesium depletion in primary cultures of hippocampal neurons. Cooper DS Eur. J. Neurosci. 29(3), 437-46, (2009)

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Sage tea drinking improves lipid profile and antioxidant defences in humans. Sá CM Int. J. Mol. Sci. 10(9), 3937-50, (2009)

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Short communication: Methamphetamine treatment increases in vitro and in vivo HIV replication. Toussi SS AIDS Res. Hum. Retroviruses 25(11), 1117-21, (2009)

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ERK regulates renal cell proliferation and renal cyst expansion in inv mutant mice. Okumura Y Acta Histochem. Cytochem. 42(2), 39-45, (2009)

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