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B7405 Sigma

Monoclonal Anti-Digoxin−Biotin antibody produced in mouse

clone DI-22, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Digoxin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies to Digoxin, DE-DL, Molecular Biology,
biological source   mouse
antibody form   purified immunoglobulin
clone   DI-22, monoclonal
form   buffered aqueous solution
application(s)   indirect ELISA: 1:10,000 using digoxin-labeled biomolecule
  western blot: 1:500 using using digoxin-labeled biomolecule
isotype   IgG1
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Digoxin-KLH.

Specificity

Antibody is specific for digoxin and shows a high affinity for digoxigenin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Application

Monoclonal Anti-Digoxin-Biotin antibody produced in mouse may be used for ELISA at a dilution of 1:10,000 and for dot blots at a dilution of 1:500. It was also used to stain apoptotic cells in cervical carcinoma sections by TUNEL technique.

Biochem/physiol Actions

Digoxin is a phytoestrogen that binds estrogen receptors and produces inotropic effects in cardiac muscle. Studies show that digoxin decreases the risk of breast and uterus cancers that are estrogen sensitive.

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
15

References

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