CelLytic™ B Cell Lysis Reagent
For bacterial cell lysis, standard strength
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MDL number MFCD02097909
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Popular Documents: Bulletin (PDF) | Specification Sheet (PDF)
Properties
| Related Categories | Bacterial Cell Lysis, Cell Lysis, Cell Lysis and Protein Extraction, Core Bioreagents, Life Science Reagents for Protein Expression and Purification, |
Description
Frequently Asked Questions
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Application
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
Features and Benefits
• Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
• Scalable for 1 to 25 grams of bacterial cell paste
• No interference with downstream applications such as affinity chromatography, IP, and Western blotting
• Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents
Other Notes
Detergent blend formulated in 40 mM Trizma® HCl (pH 8.0).
Legal Information
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Price and Availability
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≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution
For bacterial cell lysis, 2× concentrate
For bacterial cell lysis, 10× concentrate
For bacterial lysis
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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