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B7554 Sigma

Monoclonal Anti-c-Myc−Biotin antibody produced in mouse

clone 9E10, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-c-Myc

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   human
application(s)   western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein
clone   9E10, monoclonal
concentration   .8 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MYC(4609)
biological source   mouse
conjugate   biotin conjugate

Description

Immunogen

synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.

General description

The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Specificity

Specificity: N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles

Articles

Antibodies to Peptides and Fusion Tags

Unlike DNA and RNA, which can be targeted with complementary oligonucleotides, protein detection and purification usually relies upon specific antibodies. Although many are available commercially, no...
Leigh Gaskill
BioFiles 2008, 3.6, 14.
Keywords: Biofiles, Immunofluorescence, PAGE, Purification, Sequences, Western blot

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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