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B7554 Sigma

Monoclonal Anti-c-Myc−Biotin antibody produced in mouse

clone 9E10, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-c-Myc

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   human
application(s)   western blot (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells or E. coli cells expressing c-Myc tagged fusion protein
clone   9E10, monoclonal
concentration   .8 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... MYC(4609)
biological source   mouse
conjugate   biotin conjugate

Description

Immunogen

synthetic peptide corresponding to residues 408-439 of the human p62c-Myc protein.

General description

The Monoclonal Anti-c-Myc mouse antibody is isolated from ascites and conjugated to biotin. The antibody recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C terminal to the fusion protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Specificity

Specificity: N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435).

Application

The antibody is suitable for immunoblotting.

Features and Benefits

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