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B7653 Sigma

Monoclonal Anti-Biotin antibody produced in mouse

clone BN-34, ascites fluid

Synonym: Monoclonal Anti-Biotin



Related Categories Alphabetical Index, Antibodies, Antibodies to Biotin, B-BI, Biotin and Biotin Antibodies,
biological source   mouse
antibody form   ascites fluid
clone   BN-34, monoclonal
contains   15 mM sodium azide
application(s)   capture ELISA: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  indirect ELISA: 1:4,000
isotype   IgG1
conjugate   unconjugated
Featured Industry   Research Pathology
shipped in   dry ice
storage temp.   −20°C



Biotin-KLH conjugate

Biotinylated keyhole limpet hemocyanin (KLH)

Preparation Note

Developed in BALB/c mice immunized with biotinylated KLH. Mouse myeloma cells and splenocytes are fused together to form a BN-34 hybridoma.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Utilized in a variety of applications including blotting, immunocytochemistyr, insitu nucleic acid hybridization, ELISA, fluorescent activated cell-sorting (FACS) and electron microscopy.Titer is approximately 1:2000 by ELISA but should be optimized by the individual user for their application of choiceAn essential vitamin, biotin is required by living organisms and cells in culture. Avidin′s high affinity for biotin is often exploited in immuno-based assays. While standard assay methods using the avidin-biotin enzyme complex will suffice for most studies, there are occasions when enhanced sensitivity is needed to detect smaller amounts of antigen or localize low density antigens in histologic sections. Conventional immunoassay methods are improved by the use of Monoclonal Anti-Biotin which enhances the sensitivity of avidin-biotin immunoassays by bridging a second layer of avidin-biotin-enzyme complex. This antibody can be used in many other applications where biotin can be introduced as a target label.
Monoclonal Anti-Biotin antibody produced in mouse is suitable for ELISA at a working dilution of 1:4000 and for western blotting.

General description

Monoclonal Anti-Biotin recognizes the free biotin molecule and biotin conjugated to various immunoglobulins in ELISA and immunohistochemical techniques. Specificity was verified by using biotinylated goat antibodies reactive against human and rabbit antigens coated on microwell plates.

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

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NONH for all modes of transport
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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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