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  • B7661 - Monoclonal Anti-Phosphothreonine−Biotin antibody produced in mouse

B7661 Sigma

Monoclonal Anti-Phosphothreonine−Biotin antibody produced in mouse

clone PTR-8, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Phosphothreonine, Phospho Thr, Phospho Threonine, Phospho-Thr, Phospho-Threonine, p-Thr



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins,
biological source   mouse
antibody form   purified immunoglobulin
clone   PTR-8, monoclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:40,000 using phosphothreonine-BSA at 10 μg/ml, and using ExtrAvidin-HRP at 2 μg/ml
  dot blot: 1:120,000
isotype   IgG2b
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C



phosphothreonine conjugated to keyhole limpet hemocyanin (KLH).

Physical form

Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide

General description

As determined by ELISA and dot blot, the antibody reacts specifically with phosphorylated threonine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated threonine, phosphoserine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphothreonine-containing proteins. Certain proteins known to contain phosphorylated threonine may not be recognized by this antibody due to steric hindrance of the recognition site.

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Safety Information

NONH for all modes of transport
WGK Germany 
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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