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B7786 Sigma

Anti-fd Bacteriophage antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-fd Bacteriophage

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Properties

Related Categories Alphabetical Index, Antibodies, FB-FJ, Miscellaneous Antibodies, Miscellaneous Antibodies for Molecular Biology,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
form   buffered aqueous solution
application(s)   indirect ELISA: 1:1,000 using 5 × 107 phage/ml or 5 × 1010 phage/ml, respectively
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

fd bacteriophage

Preparation Note

Anti-fd Bacteriophage is developed in rabbit using repeated injections of fd bacteriophage as the immunogen. Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum which is essentially free of other rabbit serum proteins.

Specificity

The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Application

Anti-fd Bacteriophage antibody produced in rabbit may be used in ELISA at a dilution of 1:1000. It was used to in antiphage immunohistochemistry.

B7786 Rabbit Anti-fd Bactieriophage may be used to sort phage display libraries (antibody, peptide, etc.) with expressed proteins fused to either the gene III or gene VIII protein of filamentous bacteriophage. The product may also be used in "phage ELISA" offering specific activity for detection of recombinant bacteriophage. It will also function as a capture antibody when coated directly on microwell plates or as primary detection antibody for specifically captured fd or M13 bacteriophage.

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RIDADR 
NONH for all modes of transport
WGK Germany 
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