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B7931 Sigma

Anti-BNIP3 antibody, Mouse monoclonal

clone ANa40, purified from hybridoma cell culture

Synonym: Monoclonal Anti-BNIP3 antibody produced in mouse

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Bcl-2 Family Proteins,
species reactivity   human
application(s)   immunocytochemistry1: suitable
  immunoprecipitation2: suitable
  indirect ELISA1: suitable
  microarray: suitable
  western blot1: 2-4 μg/mL using whole extract of transfected 293T (human embryonal kidney) cells expressing human BNIP3.
clone   ANa40, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG2b
mol wt   antigen mol wt 21.5 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BNIP3(664)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant human BNIP3 (amino acids 1-163, BNIP3ΔTM2).

General description

BNIP3, formerly NIP3 (nineteen kDa interacting protein-3), is a pro-apoptotic mitochondrial protein classified in the Bcl-2 family based on limited sequence homology to the Bcl-2 homology 3 (BH3) domain (amino acids 110-118) and C-terminal TM domain. BNIP3 expressed in yeast and mammalian cells interacts with survival promoting proteins Bcl-2, Bcl-XL, CED-9 and the adenovirus E1B 19K protein. BNIP3 represents a subfamily of Bcl-2-related proteins, which functions without a typical BH3 domain to regulate apoptosis from both mitochondrial and non-mitochondrial sites by selective Bcl-2/Bcl-XL interactions. Endogenous BNIP3 is loosely associated with mitochondrial membrane in normal tissue but fully integrates into the mitochondrial outer membrane with the N-terminus in the cytoplasm and the C-terminus in the membrane during induction of cell death. This is accompanied by rapid and profound mitochondrial dysfunction characterized by opening of the mitochondrial permeability transition (PT) pore, proton electrochemical gradient (Dym) suppression, and increased reactive oxygen species production.

Rabbit monoclonal clone ANa40 anti-BNIP3 recognizes human BNIP3. The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Rabbit monoclonal clone ANa40 anti-BNIP3 is used to tag BNIP3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It is used as a probe to determine the presence and roles of BNIP3 in the regulation of mitochondrial and non-mitochondrial mediated apoptosis.

The product is useful in ELISA, immunoblotting (21.5 kDa, apparent molecular mass in SDS-PAGE is approximately 30 and 60 kDa, for monomer and dimer, respectively), immunocytochemistry (4% formaldehyde), and immunoprecipitation.

Price and Availability


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