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B8400 Sigma

Monoclonal Anti-Bovine IgG2 antibody produced in mouse

clone BG2-7, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, BL-BZ, Bovine Secondary Antibodies and Conjugates, Primary Antibodies,
biological source   mouse
antibody form   ascites fluid
clone   BG2-7, monoclonal
contains   15 mM sodium azide
application(s)   capture ELISA: suitable
  dot blot: suitable
  indirect ELISA: 1:1,000
  western blot: suitable
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG2 antibody is suitable for use in western blot, indirect ELISA (1:1,000), dot blot and capture ELISA.

General description

Bovine IgGs are glycoprotein antibodies that regulate immune responses in herds. These antibodies inhibit TLR5 activation upon immunization with native H7 flagellin . Bovine IgG levels can be used for the detection of Johne′s disease and milk adulteration . Monoclonal Anti-Bovine IgG2 antibody is specific for an epitope on the heavy chain of bovine IgG2. The antibody does not bind to bovine light chains, IgG1, and IgM in their native form or SDS-reduced and denatured forms. As determined by ELISA, the product does not cross-react with bovine IgM or with IgGs from the following species: human, goat, sheep, rabbit, pig, dog, chicken, cat, guinea pig or horse.

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Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

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Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
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References

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