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B8416 Sigma

Bleomycin sulfate from Streptomyces verticillus

1.5-2.0 units/mg solid, BioReagent, suitable for cell culture

Synonym: Blenoxane, Bleo, Blexane



This product is a mixture of glycopeptides antibiotics containing Bleomycin A2 (70%) and B2(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.


Bleomycin Sulfate is an atineoplastic antibiotic isolated from Streptomyces verticillus. It is recommended for use as a cell culture agent at 10-100 μg/mL.

Biochem/physiol Actions

Mode of Action: Bleomycin Sulfate binds to DNA, inhibits DNA synthesis, and causes single strand DNA scission at specific base sequences. This antibiotic can also cleave RNA, to a lesser degree but more selectively, and ats as an inducer and regulator of apoptosis and inhibits tumor angiogenesis. To cleave DNA, it is required to bind oxygen and a metal ion.


This product should be stored at 2-8°C.

Preparation Note

The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.

Protocols & Applications

Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

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