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B8429 Sigma

Anti-Bax antibody, Mouse monoclonal

clone 6A7, purified from hybridoma cell culture

Synonym: Monoclonal Anti-Bax antibody produced in mouse

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   mouse, rat, human
application(s)   immunoprecipitation: suitable
  microarray: suitable
  western blot: 10-60 μg/mL using extract of human breast adenocarcinoma MCF-7 cells, activated with dexamethasone
clone   6A7, monoclonal
concentration   2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 21 kDa (and possibly a dimer of 42 kDa)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BAX(581)
mouse ... Bax(12028)
rat ... Bax(24887)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 12-24 of the human Bax sequence, conjugated to KLH.

General description

Bax is a 21kD integral organelle membrane protein that belongs to the Bcl-2 family and is expressed mainly in the mitochondria. The formation of bax homodimer accelerates apoptotic death whereas its heterodimerization with Bcl-2 or Bcl-xL can block apoptosis. Monoclonal anti-Bax antibody can be used to study the intracellular redistribution of Bax protein upon induction of apoptosis and its unique subcellular localization. This product can also be used in immunoblot analysis to estimate variations in the expression of specific proteins involved in apoptosis signalling. Mouse anti-Bax antibody reacts specifically with the epitope present within the amino acids 12-24 shared by bax from rat, mouse and human.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Monoclonal anti-Bax antibody can be used in immunocytochemistry (diluted 1: 1500) and western blot. It is also useful in microarray and immunoprecipitation.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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