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B8434 Sigma

Monoclonal Anti-BrdU antibody produced in mouse

~1.5 mg/mL, clone BU-33, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Bromodeoxyuridine

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nucleic Acids and Nucleic Acid Metabolism,
biological source   mouse
antibody form   purified immunoglobulin
clone   BU-33, monoclonal
form   buffered aqueous solution
species reactivity   wide range
concentration   ~1.5 mg/mL
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

bromodeoxyuridine conjugated to KLH.

Specificity

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Price and Availability

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Safety & Documentation

Safety Information

WGK Germany 
nwg

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, High performance liquid chromatography, Ion Exchange, Microscopy, Precipitation, Purification, Scanning electron microscopy

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Peer-Reviewed Papers

References

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G-protein-coupled OX1 orexin/hcrtr-1 hypocretin receptors induce caspase-dependent and -independent cell death through p38 mitogen-/stress-activated protein kinase. Akerman, K., et al. J. Biol. Chem. 281, 834-842, (2006)

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New chromogens for alkaline phosphatase histochemistry: salmon and magenta phosphate are useful for single- and double-label immunohistochemistry. Avivi, C., et al. J. Histochem. Cytochem. 42, 551-554, (1994)

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Selective impairments to memory consolidation in chicks produced by 5'-iodo-2'-deoxyuridine. Komissarova, N.V., et al. Neurosci. Behav. Physiol. 40, 215-23, (2010)

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Replication stress induces 53BP1-containing OPT domains in G1 cells. Harrigan, J.A., et al. J. Cell Biol. 193, 97-108, (2011)

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Huperzine A activates Wnt/β-catenin signaling and enhances the nonamyloidogenic pathway in an Alzheimer transgenic mouse model. Wang, C.Y., et al. Neuropsychopharmacology 36, 1073-89, (2011)

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Ezh2, the histone methyltransferase of PRC2, regulates the balance between self-renewal and differentiation in the cerebral cortex. Pereira, J.D., et al. Proc. Natl. Acad. Sci. U. S. A. 107, 15957-62, (2010)

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Cyst Formation And Activation Of The Extracellular Regulated Kinase Pathway After Kidney Specific Inactivation Of Pkd1. Shibazaki, S., et al. Hum. Mol. Genet. 17, 1505-16, (2008)

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Developmental Links Between The Fetal And Adult Zones Of The Adrenal Cortex Revealed By Lineage Tracing. Zubair, M., et al. Mol. Cell. Endocrinol. 28, 7030-40, (2008)

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The Bmi-1 Polycomb Protein Antagonizes The (–)-epigallocatechin-3-gallate-dependent Suppression Of Skin Cancer Cell Survival Balasubramanian, S., et al. Carcinogenesis 31, 496 - 503, (2010)

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Mouse embryos stressed by physiological levels of osmolarity become arrested in the late 2-cell stage before entry into M phase. Wang, F., et al. Biol. Reprod. 85, 702-13, (2011)

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A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine. Kotogány, E., et al. Plant Methods 6, 5, (2010)

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Axon-bearing and axon-less horizontal cell subtypes are generated consecutively during chick retinal development from progenitors that are sensitive to follistatin Hallbook, F., et al. BMC Dev. Biol. 8, 46, (2008)

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The role of RXFP2 in mediating androgen-induced inguinoscrotal testis descent in LH receptor knockout mice. Yuan, F.P., et al. Reproduction 139, 759-69, (2010)

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Accumulation Of A Differentiation Regulator Specifies Transit Amplifying Division Number In An Adult Stem Cell Lineage Insco, M., et al. PLoS Pathog. 106, 22311 - 22316, (2009)

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The heparin-binding domain of IGFBP-2 has insulin-like growth factor binding-independent biologic activity in the growing skeleton. Kawai, M., et al. J. Biol. Chem. 286, 14670-80, (2011)

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Transgenic Expression Of Ad4BP/SF-1 In Fetal Adrenal Progenitor Cells Leads To Ectopic Adrenal Formation. Zubair, M., et al. Mol. Cancer Ther. 23, 1657-67, (2009)

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Protein kinase C (PKC) delta suppresses keratinocyte proliferation by increasing p21(Cip1) level by a KLF4 transcription factor-dependent mechanism. Chew, Y.C., et al. J. Biol. Chem. 286, 28772-82, (2011)

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Decreased MCM2-6 in Drosophila S2 cells does not generate significant DNA damage or cause a marked increase in sensitivity to replication interference. Crevel, I., et al. PLoS ONE 6, e27101, (2011)

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ERG oncogene modulates prostaglandin signaling in prostate cancer cells. Mohamed, A.A., et al. Cancer Biol. Ther. 11, 410-7, (2011)

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Identification of novel 1,4-benzoxazine compounds that are protective in tissue culture and in vivo models of neurodegeneration. Wang, L., et al. J. Neurosci. Res. 88, 1970-84, (2010)

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Olfactory ensheathing cells promote proliferation and inhibit neuronal differentiation of neural progenitor cells through activation of Notch signaling Chen, B., et al. Neuroscience 153, 406-413, (2008)

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Reducing hippocampal cell proliferation in the adult rat does not prevent the acquisition of cocaine-induced conditioned place preference. Brown, T.E., et al. Neurosci. Lett. 481, 41-6, (2010)

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The CDK9/cyclin T1 subunits of P-TEFb in mouse oocytes and preimplantation embryos: a possible role in embryonic genome activation. Oqani, R.K., et al. BMC Dev. Biol. 11, 33, (2011)

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Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine-serine-rich (RS) domains of SR- and SR-like proteins. Heim A, Grimm C, Müller U, et al. Nucleic Acids Res. 42(12), 7833-50, (2014)

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Spontaneous in vitro senescence of glioma cells confirmed by an antibody against IDH1R132H. Stoczynska-Fidelus E, Och W, Rieske P, et al. Anticancer Res. 34(6), 2859-67, (2014)

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Hydrogen peroxide primes heart regeneration with a derepression mechanism. Han P, Zhou XH, Chang N, et al. Cell Res. 24(9), 1091-107, (2014)

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Sohlh2 inhibits ovarian cancer cell proliferation by upregulation of p21 and downregulation of cyclin D1. Zhang H, Zhang X, Ji S, et al. Carcinogenesis 35(8), 1863-71, (2014)

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