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B8434 Sigma

Anti-BrdU antibody, Mouse monoclonal

clone BU-33, purified from hybridoma cell culture

Synonym: Anti-Bromodeoxyuridine, Monoclonal Anti-BrdU antibody produced in mouse

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nucleic Acids and Nucleic Acid Metabolism,
biological source   mouse
antibody form   purified immunoglobulin
clone   BU-33, monoclonal
form   buffered aqueous solution
species reactivity   wide range
concentration   ~1.5 mg/mL
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

bromodeoxyuridine conjugated to KLH.

Packaging

200 μL in glass bottle

Specificity

Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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