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  • B8434 - Monoclonal Anti-BrdU antibody produced in mouse


B8434 Sigma

Monoclonal Anti-BrdU antibody produced in mouse

~1.5 mg/mL, clone BU-33, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-Bromodeoxyuridine



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nucleic Acids and Nucleic Acid Metabolism,
antibody form   purified immunoglobulin
clone   BU-33, monoclonal
form   buffered aqueous solution
species reactivity   wide range
concentration   ~1.5 mg/mL
application(s)   immunocytochemistry: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1-2 μg/mL using sections of intestine from mice or rats treated in vivo with BrdU
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C



bromodeoxyuridine conjugated to KLH.


Reacts specifically with BrdU (bromodeoxyuridine) incorporated into DNA or coupled to a protein carrier. Cross-reaction with IdU (iododeoxyuridine) is observed.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium axide.

Price and Availability

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Safety & Documentation

Safety Information

WGK Germany 

Protocols & Articles

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Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot

Peer-Reviewed Papers


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Replication stress induces 53BP1-containing OPT domains in G1 cells. Harrigan, J.A., et al. J. Cell Biol. 193, 97-108, (2011)


Cyst Formation And Activation Of The Extracellular Regulated Kinase Pathway After Kidney Specific Inactivation Of Pkd1. Shibazaki, S., et al. Hum. Mol. Genet. 17, 1505-16, (2008)


The heparin-binding domain of IGFBP-2 has insulin-like growth factor binding-independent biologic activity in the growing skeleton. Kawai, M., et al. J. Biol. Chem. 286, 14670-80, (2011)


The Bmi-1 Polycomb Protein Antagonizes The (–)-epigallocatechin-3-gallate-dependent Suppression Of Skin Cancer Cell Survival Balasubramanian, S., et al. Carcinogenesis 31, 496 - 503, (2010)


G-protein-coupled OX1 orexin/hcrtr-1 hypocretin receptors induce caspase-dependent and -independent cell death through p38 mitogen-/stress-activated protein kinase. Akerman, K., et al. J. Biol. Chem. 281, 834-842, (2006)


Protein kinase C (PKC) delta suppresses keratinocyte proliferation by increasing p21(Cip1) level by a KLF4 transcription factor-dependent mechanism. Chew, Y.C., et al. J. Biol. Chem. 286, 28772-82, (2011)


Mouse embryos stressed by physiological levels of osmolarity become arrested in the late 2-cell stage before entry into M phase. Wang, F., et al. Biol. Reprod. 85, 702-13, (2011)


New chromogens for alkaline phosphatase histochemistry: salmon and magenta phosphate are useful for single- and double-label immunohistochemistry. Avivi, C., et al. J. Histochem. Cytochem. 42, 551-554, (1994)


Selective impairments to memory consolidation in chicks produced by 5'-iodo-2'-deoxyuridine. Komissarova, N.V., et al. Neurosci. Behav. Physiol. 40, 215-23, (2010)


Ezh2, the histone methyltransferase of PRC2, regulates the balance between self-renewal and differentiation in the cerebral cortex. Pereira, J.D., et al. Proc. Natl. Acad. Sci. U. S. A. 107, 15957-62, (2010)


Huperzine A activates Wnt/β-catenin signaling and enhances the nonamyloidogenic pathway in an Alzheimer transgenic mouse model. Wang, C.Y., et al. Neuropsychopharmacology 36, 1073-89, (2011)


Axon-bearing and axon-less horizontal cell subtypes are generated consecutively during chick retinal development from progenitors that are sensitive to follistatin Hallbook, F., et al. BMC Dev. Biol. 8, 46, (2008)


A rapid and robust assay for detection of S-phase cell cycle progression in plant cells and tissues by using ethynyl deoxyuridine. Kotogány, E., et al. Plant Methods 6, 5, (2010)


Identification of novel 1,4-benzoxazine compounds that are protective in tissue culture and in vivo models of neurodegeneration. Wang, L., et al. J. Neurosci. Res. 88, 1970-84, (2010)


Accumulation Of A Differentiation Regulator Specifies Transit Amplifying Division Number In An Adult Stem Cell Lineage Insco, M., et al. PLoS Pathog. 106, 22311 - 22316, (2009)


Developmental Links Between The Fetal And Adult Zones Of The Adrenal Cortex Revealed By Lineage Tracing. Zubair, M., et al. Mol. Cell. Endocrinol. 28, 7030-40, (2008)


Reducing hippocampal cell proliferation in the adult rat does not prevent the acquisition of cocaine-induced conditioned place preference. Brown, T.E., et al. Neurosci. Lett. 481, 41-6, (2010)


The role of RXFP2 in mediating androgen-induced inguinoscrotal testis descent in LH receptor knockout mice. Yuan, F.P., et al. Reproduction 139, 759-69, (2010)


Jumonji domain containing protein 6 (Jmjd6) modulates splicing and specifically interacts with arginine-serine-rich (RS) domains of SR- and SR-like proteins. Heim A, Grimm C, Müller U, et al. Nucleic Acids Res. 42(12), 7833-50, (2014)


Transgenic Expression Of Ad4BP/SF-1 In Fetal Adrenal Progenitor Cells Leads To Ectopic Adrenal Formation. Zubair, M., et al. Mol. Cancer Ther. 23, 1657-67, (2009)


Olfactory ensheathing cells promote proliferation and inhibit neuronal differentiation of neural progenitor cells through activation of Notch signaling Chen, B., et al. Neuroscience 153, 406-413, (2008)


Spontaneous in vitro senescence of glioma cells confirmed by an antibody against IDH1R132H. Stoczynska-Fidelus E, Och W, Rieske P, et al. Anticancer Res. 34(6), 2859-67, (2014)


ERG oncogene modulates prostaglandin signaling in prostate cancer cells. Mohamed, A.A., et al. Cancer Biol. Ther. 11, 410-7, (2011)


Hydrogen peroxide primes heart regeneration with a derepression mechanism. Han P, Zhou XH, Chang N, et al. Cell Res. 24(9), 1091-107, (2014)


Sohlh2 inhibits ovarian cancer cell proliferation by upregulation of p21 and downregulation of cyclin D1. Zhang H, Zhang X, Ji S, et al. Carcinogenesis 35(8), 1863-71, (2014)


Decreased MCM2-6 in Drosophila S2 cells does not generate significant DNA damage or cause a marked increase in sensitivity to replication interference. Crevel, I., et al. PLoS ONE 6, e27101, (2011)


The CDK9/cyclin T1 subunits of P-TEFb in mouse oocytes and preimplantation embryos: a possible role in embryonic genome activation. Oqani, R.K., et al. BMC Dev. Biol. 11, 33, (2011)


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