• USA Home
  • B8781 - Restriction Enzyme Buffer SB

EMAIL THIS PAGE TO A FRIEND
B8781 Sigma

Restriction Enzyme Buffer SB

10 ×

Synonym: Restriction Endonuclease Buffer

Purchase

Properties

Related Categories Buffers for Restriction Enzymes, Molecular Biology, Molecular Biology Enzymes More...
grade   for molecular biology
description   10× buffer contains 100 mM Tris-HCl, pH 8.0, 50 mM MgCl2, 1000 mM NaCl, 10 mM 2-mercaptoethanol at 37 °C.
InChI Key   YNZJHLFHFNCZIE-UHFFFAOYSA-N
form   buffered aqueous solution
concentration   10 ×
storage temp.   −20°C

Description

General description

This buffer is for use with Sigma Restriction Enzymes. Please note every restriction enzyme is supplied with the appropriate buffer - this product is available for users who need additional buffer. For details on buffer composition and protocols for use, see the "Protocols and Articles" tab above.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Restriction Enzyme Buffer Reference

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. Reference below the enzyme activity in each buffer (table 1), and the preferred buffer ...
Keywords: Buffers, Digestions, Enzyme activity, PAGE, Solid phase extractions

Protocols

Restriction Endonucleases - The Molecular Scissors

The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson. The ability of certain E. coli strains to ...
Keywords: Asymmetric synthesis, Buffers, Cloning, Degradations, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetic, Indirect Immunofluorescense, Methylations, Molecular biology, Molecular biology techniques, Peptide synthesis, Polymorphisms, Reproduction, Separation, Sequencing

Restriction Enzyme Digest Protocol

Sigma® Life Science’s restriction enzyme collection has been optimized for digestion using five unique buffers. When digesting DNA using a single enzyme, use the buffer supplied with the enzyme (also...
Keywords: Buffers, Digestions, Purification

Related Products

related product

Product #

Description

Add to Cart

R0260 BamH I from Bacillus amyloliquefaciens H, Restriction Enzyme
R1137 Hind III from Haemophilus influenzae, Restriction Enzyme
R2756 EcoR V from Escherichia coli, buffered aqueous glycerol solution

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?