• USA Home
  • B8895 - Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat

EMAIL THIS PAGE TO A FRIEND
B8895 Sigma

Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Biotin Antibodies, Primary Antibodies, RAB-RAD,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:200,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800
  western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
conjugate   biotin conjugate
storage temp.   −20°C

Description

Other Notes

Antibody adsorbed with human IgG.

Immunogen

Purified rabbit IgG

Preparation Note

Adsorbed to reduce background staining with human samples.

Specificity

Binds all rabbit Igs.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide

Application

Anti-Rabbit IgG (whole molecule)-Biotin antibody is suitable for use in immunofluorescence (1:800) and immunohistochemistry. The product can also be used for western blot (5-10 μg per well at 1:400,000-1:800,000 dilutions using total cell extract of HeLa cells) and direct ELISA (1:200,000).

Polyclonal anti-rabbit IgG (whole molecule)-biotin antibody can be used in immunofluorescence analysis and immunocytochemistry.

General description

IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG.

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?