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  • B8895 - Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat

B8895 Sigma

Anti-Rabbit IgG (whole molecule)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Biotin Antibodies, Primary Antibodies, RAB-RAD,
biological source   goat
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:200,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800
  western blot: 1:400,000-1:800,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)
conjugate   biotin conjugate
storage temp.   −20°C


Other Notes

Antibody adsorbed with human IgG.


Purified rabbit IgG

Preparation Note

Adsorbed to reduce background staining with human samples.


Binds all rabbit Igs.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide


Anti-Rabbit IgG (whole molecule)-Biotin antibody is suitable for use in immunofluorescence (1:800) and immunohistochemistry. The product can also be used for western blot (5-10 μg per well at 1:400,000-1:800,000 dilutions using total cell extract of HeLa cells) and direct ELISA (1:200,000).

Polyclonal anti-rabbit IgG (whole molecule)-biotin antibody can be used in immunofluorescence analysis and immunocytochemistry.

General description

IgG antibodies regulate several functions such as phagocytosis and complement activation. Thus they play a crucial role in facilitating cytological immune responses. Anti-rabbit IgG (whole molecule)–biotin antibody can be used in immunohistochemistry. The product shows minimal interspecies cross reactivity to human IgG hence can be used in enzyme immunoassays or dot blot in presence of human IgG. Anti-rabbit IgG (whole molecule)–biotin antibody reacts specifically to all rabbit immunoglobulin but has no reactivity with human IgG.

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins
Anti-Rabbit IgG (whole molecule)-Biotin antibody reacts with rabbit serum and rabbit IgG. The product does not cross-react with human IgG.

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


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92210 Timestrip Plus 0 °C
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