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B8931 Sigma

Magenta-Gal

reagent for selection of recombinant bacterial clones

Synonym: 5-Bromo-6-chloro-3-indolyl β-D-galactopyranoside, Red-Gal

  • CAS Number 93863-88-8

  • Empirical Formula (Hill Notation) C14H15BrClNO6

  • Molecular Weight 408.63

  •  MDL number MFCD02683897

Purchase

Properties

Related Categories Chromogenic, Galactoside, Glycobiology, Inhibitors and Substrates, Molecular Biology,
grade   for molecular biology
InChI Key   CHRVKCMQIZYLNM-HTOAHKCRSA-N
sterility   non-sterile
product line   BioReagent
assay   ≥98.0% (HPLC)
form   powder
solubility   methanol: soluble
suitability   suitable for substrate for β-galactosidase
storage temp.   −20°C

Description

Application

Chromogenic substrate for β-galactosidase producing a red or magenta insoluble product.

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype.

General description

Red-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Red-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Principle

When Red-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Red-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Price and Availability


Detect Your Unseen Protein
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

Peer-Reviewed Papers
15

References

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