• USA Home
  • B9146 - 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

EMAIL THIS PAGE TO A FRIEND
B9146 Sigma

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

≥98%

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

Purchase

Properties

Related Categories Chromogenic, Galactoside, Gene Reporter Substrates, Glycobiology, Inhibitors and Substrates,
InChI Key   OPIFSICVWOWJMJ-AEOCFKNESA-N
assay   ≥98%
suitability   suitable for identification of lac+ bacterial colonies
storage temp.   −20°C

Description

Application

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Packaging

10, 100, 500 mg in poly bottle

Reconstitution

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20 °C and protected from light. Sterilization is not required.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Competent Cells

Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment. Once it enters the cytoplasm, the genetic material may be degraded by nucleases i...
Keywords: Antibiotics, Cloning, Detergents, Electrophoresis, Gel electrophoresis, Genetic, Molecular biology, Peptide synthesis, transformation

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Introduction to Yeast Transformation

Transformation is the process by which exogenous DNA is introduced into a cell, resulting in an inheritable change or genetic modification. This was first reported in Streptococcus pneumoniae by Grif...
Keywords: Cell disruption, Food & Beverage, Gene expression, Genetic, Molecular biology, Pharmaceutical, Polymerase chain reaction, transformation

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Transformation Protocols

From our library of Protocols, Sigma-Aldrich presents Transformation Protocols
Keywords: Antibiotics, Detergents, Electrophoresis, Gel electrophoresis, Mass spectrometry, Peptide synthesis, transformation

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Degradations, Genetic, Molecular biology, Transfection, transformation

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

related product

Product #

Description

Add to Cart

94433 β-Galactosidase stain
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?