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B9428 Sigma

Monoclonal Anti-Bin1 antibody produced in mouse

clone 99D, ascites fluid

Synonym: Anti-Amphiphysin II, Anti-SH3P9, Anti-ampl

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   human, mouse, rat, avian, rabbit
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable using nucleoplasm, subnuclear punctate compartment, and cytoplasm
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 1:50,000 using a rat brain cytosol preparation
clone   99D, monoclonal
antibody form   ascites fluid
isotype   IgG2b
mol wt   antigen mol wt 65-75 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BIN1(274)
mouse ... Bin1(30948)
rat ... Bin1(117028)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant polypeptide containing amino acids 189-398 of human Bin1.

Specificity

The antibody reacts specifically with a 33-residue segment of the myc-binding domain (MBD, amino acids 323-356) of the human Bin1 molecule. It does not react with the Bin1-related 45 kDa polypeptide.

Price and Availability


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