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B9655 Sigma

Monoclonal Anti-Avidin–Biotin antibody produced in mouse

clone WC19.10, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Avidin

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Properties

Related Categories ATH-AZ, Alphabetical Index, Antibodies, Miscellaneous, Primary Antibodies More...
biological source   mouse
antibody form   purified immunoglobulin
clone   WC19.10, monoclonal
form   buffered aqueous solution
application(s)   direct ELISA: suitable
  dot blot: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
isotype   IgG1
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Immunogen

Purified egg white avidin.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

Application

Biotin conjugated Monoclonal Anti-Avidin may be used for amplification of biotin-avidin reactions (e.g., immunohistochemistry, in situ hybridization).

Monoclonal Anti-Avidin-Biotin antibody produced in mouse may be used for ELISA at a working dilution of 1:150,000; for dot blot at a working dilution of 1:20,000; for immunohistochemistry at a working dilution of 1:2000. It was used for FISH analysis of chromosomes X and 8 in lymphocytes isolated from women.

General description

The antibody binds to the avidin molecule without interfering with the biotin-binding reaction. The product reacts specifically with egg white avidin, ExtrAvidin and their complexes formed with biotin. The antibody shows no cross-reaction with streptavidin.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

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Peer-Reviewed Papers
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References

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