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B9673 Sigma

1-Bromo-3-chloropropane

for isolation of RNA

Synonym: 1-Bromo-3-chloropropane, I-BCP, Trimethylene bromochloride, Trimethylene chlorobromide

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Properties

Related Categories Biochemicals, Molecular Biology, Molecular Biology Reagents More...
grade   for molecular biology
InChI Key   MFESCIUQSIBMSM-UHFFFAOYSA-N
assay   ≥98.5% (GC)
form   liquid
refractive index   n20/D 1.486(lit.)
bp   144-145 °C(lit.)
density   1.592 g/mL at 25 °C(lit.)
storage temp.   room temp

Description

Packaging

200 mL in glass bottle

Application

Suitable for isolation of high quality RNA for molecular biology applications.

Features and Benefits

• less toxic alternative to chloroform
• compatible with TRI reagent for isolation of RNA with decreased DNA contamination

General description

1-Bromo-3-chloropropane is a phase separation reagent used in protocols for isolation of nucleic acids.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
RIDADR 
UN 2688 6.1 / PGIII
WGK Germany 
3
RTECS 
TX4113000
Flash Point(F) 
>284 °F
Flash Point(C) 
>140 °C
Protocols & Articles

Protocols

MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting
Keywords: AGE, Amplification, Buffers, Cell disruption, Centrifugation, Degradations, Diagnostic, Digestions, Diseases, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Microarray Analysis, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Sample preparations, Separation, Solvents, Titrations

TRI Reagent® Protocol

1A. Tissue: Homogenize tissue samples in TRI Reagent (1 ml per 50–100 mg of tissue) in a Polytron® or other appropriate homogenizer.
Keywords: AGE, Buffers, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Homogenization, PAGE, Polymerase chain reaction, Precipitation, Sample preparations, Separation, Solvents, Western blot

Peer-Reviewed Papers
15

References

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Description

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93289 TRI Reagent®, for DNA, RNA and protein isolation

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