• USA Home
  • B9780 - Monoclonal Anti-Bovine IgG−Biotin antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
B9780 Sigma

Monoclonal Anti-Bovine IgG−Biotin antibody produced in mouse

clone BG-18, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Bovine IgG

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, BL-BZ, Biotin Antibodies, Bovine Secondary Antibodies and Conjugates,
biological source   mouse
antibody form   purified immunoglobulin
clone   BG-18, monoclonal
form   buffered aqueous solution
species reactivity   bovine
does not react with   goat, guinea pig, sheep, pig, bovine (IgM), horse, cat, rabbit, human, canine
application(s)   direct ELISA: 1:50,000
  dot blot: 1:60,000-1:120,000
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
isotype   IgG1
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C

Description

Specificity

bovine IgG1 and IgG2

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Monoclonal Anti-Bovine IgG-Biotin antibody produced in mouse may be used for ELISA at a working dilution of 1:50,000. It may be suitable for dot blot at a dilution range of 1:60,000-1:120,000. The antibody was used in ELISA to determine the antibody response to rabies vaccine in cattle.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of biotin to Anti-Bovine IgG antibody allows for the binding of various labels such as avidin or streptavidin.

General description

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer - Over 80,000 Products

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?