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BCA1 Sigma

Bicinchoninic Acid Kit for Protein Determination

for 200-1000 μg/ml protein

Synonym: BCA protein assay

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Description

Features and Benefits

• Simple, sensitive colorimetric assay for proteins
• Two stable reagents are used for the working solution
• Faster and easier than the Lowry protein assay
• Compatible with many detergents both ionic and nonionic
• Less variation between different proteins than the Bradford dye-binding assay
• Adaptable for use in microwell plates

Principle

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Price and Availability


Amplified Detection

Standard component

Description

Product #

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Bicinchoninic Acid Solution 1 L SDS    
4%(w/v) CuSO4 · 5H2O Solution 25 mL SDS    
Protein Standard Solution 5 x 1 mL SDS    
Safety & Documentation

Safety Information

Symbol 
GHS09  GHS09
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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