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BE0200 Sigma

β Amyloid 1-42 ELISA human

sufficient for 96 multiwell tests

Synonym: β Amyloid ELISA, Amyloid β ELISA

Properties

usage   sufficient for 96 multiwell tests
shipped in   wet ice
storage temp.   2-8°C

Description

Features and Benefits

The assay is a sensitive (10 pg/mL), and specific alternative to immunoblotting or RIA.

Application

Human β-amyloid (hAβ) ELISAs are designed for in vitro quantitative detection of native and recombinant β-amyloid in sera, cell culture supernatants and other biological samples. hAβ′s are main component of the protein deposits in neurofibrillary tangles, neuritic plaques and neuropil threads in the cerebral cortex of Alzheimer′s Disease (AD) and Down′s Syndrome. The antibodies used in these kits selectively differentiate between Aβ40 and Aβ42/Aβ43.

The ELISA can be used for in vitro quantitative determination of human native and recombinant β-amyloid 1-42 (hAβ42) protein in serum, cell culture supernatants and other biological samples. The Aβ42 antibody used in this kit selectively detects hAβ42 and not hAβ40.

General description

A solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal capture antibody specific for the NH2 terminus of hAβ has been coated onto the multiwell strips. hAβ antigen in standards and samples binds to the capture antibody. A detection antibody specific for the hAβ 1−40 or 1−42 sequence binds the immobilized hAβ. An anti-rabbit IgG-HRP completes the sandwich. The reaction is visualized by TMB substrate, followed by the stop solution. The intensity of the yellow color is directly proportional to the concentration of hAβ in the original sample.

Price and Availability

Kit component only

Description

Product #

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Standard hAβ42, lyophilized synthetic peptide MSDS    
Monoclonal Anti-hAβ NH2-Terminus Specific Antibody-Coated 96 well plate MSDS    
Anti-hAβ 1-42 Antibody MSDS    
Anti-Rabbit IgG-HRP Concentrate, 100X MSDS    
HRP Diluent MSDS    
Wash Buffer Concentrate, 25X MSDS    
Stabilized TMB Chromogen MSDS    
Plate Sealers MSDS    
Stop Solution MSDS    
Standrad/Sample Diluent MSDS    

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