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  • C0228 - Anti-phospho-Cdc2 (Cdk1) (pTyr15) antibody produced in rabbit

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C0228 Sigma

Anti-phospho-Cdc2 (Cdk1) (pTyr15) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human, mouse, rat
application(s)   immunoprecipitation: 1:100
  western blot (chemiluminescent): 1:1,000 using extract from hydroxyurea treated Saos cells
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CDC2(983)
mouse ... Cdc2a(12534)
rat ... Cdc2(54237)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic phosphopeptide corresponding to residues around Tyr15 of human Cdc2.

Physical form

Solution in 10 mM sodium HEPES, pH 7.5, containing 0.15 M sodium chloride, 100 μg/ml bovine serum albumin, and 50% glycerol.

Specificity

The antibody detects phosphorylated tyrosine on Cdc2 and Cdk1. It does not react with non-phosphorylated Cdc2, Cdk4, Cdk6, or Cdk7.

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