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C0354 Sigma

Anti-p130CAS antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Crk-Associated Protein (p130Cas),
species reactivity   rat, human, mouse
application(s)   immunoprecipitation: 3 μg using using Protein A-agarose and 10 μg lysate of cultured ECV304 human endothelial cells or mouse fibroblasts NIH3T3 cell lysate
  microarray: suitable
  western blot: 1:1,000 using whole extract of the human ECV304 endothelial cells or a whole extract of the rat skeletal muscle myoblasts L8 cell line
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 130 kDa
storage temp.   −20°C
Gene Information   human ... BCAR1(9564)
mouse ... Bcar1(12927)
rat ... Bcar1(25414)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

Synthetic peptide corresponding to the C-terminal region of human (p130cas) (amino acids 642-659), coupled to KLH. This sequence is identical in mouse and rat (p130cas) and has no homology to Cas-related proteins Hef1/Cas-L and Efs/Sin.

General description

p130cas or CAS (Crk-associated substrate) is a 120-130kD docking protein consists of a repetitive stretch of tyrosine-containing motifs. It has a pivotal role in cell adhesion, cell migration, cytokine receptor engagement and growth factor stimulation. It also has a critical role in cell transformation. Anti-(p130cas) antibody can be used in western blotting (diluted 1:1000) using human ECV304 endothelial cell extracts and rat skeletal muscle myoblasts L8 cell line extract. Rabbit anti-(p130cas) antibody reacts specifically with p130cas of rat, mouse and human.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-(p130cas) antibody can be used in immunoprecipitation and microarray.

Price and Availability


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WGK Germany  2
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