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  • C0478 - Anti-Calpain LP-85 (Lens-Specific Calpain) (Domain IV), Large Subunit antibody produced in rabbit

C0478 Sigma

Anti-Calpain LP-85 (Lens-Specific Calpain) (Domain IV), Large Subunit antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   rat, mouse
application(s)   western blot: 1:1,000
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   mouse ... Capn3(12335)
rat ... Capn3(29155)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to a unique insert found in the ELF-hand (the calcium binding domain-IV in the large subunit) of rat LP-85 calpain (lens-specific calpain).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 50% glycerol and 15 mM sodium azide


By immunoblotting against the reduced protein, the antibody reacts with bands at 85 kDa and 62 kDa and a series of further cleaved active forms. It also reacts with non-reduced calpain LP-85. The antibody does not cross-react with other calpain family members (calpain LP-82, μ-calpain, m-calpain, calpain-94, ncl-2, ncl-3, etc.).

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WGK Germany  1
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