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C0543 Sigma

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Type IV (Miller), lyophilized powder (from 0.2 μm-filtered solution), BioReagent, suitable for cell culture

Synonym: EHS-collagen



Related Categories Adhesion, Attachment, and Matrix Factors, Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Attachment Factors, Attachments Factors, Biochemicals and Reagents,
type   Type IV (Miller)
product line   BioReagent
form   lyophilized powder (from 0.2 μm-filtered solution)
surface coverage   6‑10 μg/cm2
solubility   0.25% acetic acid: soluble0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
suitability   suitable for cell culture
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)



Epithelial cells, endothelial cells, muscle cells, nerve cells

Recommended for use as a cell culture substratum at 6-10 μg/cm2.

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Other Notes

Native collagen


1 vial in glass bottle

Preparation Note

Chloroform treated.
Pepsin is not used in the preparation.


0.75 mg/vial

Price and Availability

All labs need water
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


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Cancer Stem Cells: New Targets for Cancer Therapy

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Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation

Collagen Attachment Protocols, Solubility, and Stability

A1) We recommend transferring the collagen solution to a glass bottle with a screw cap and carefully layering chloroform at the bottom. The amount of chloroform to use should be ~10% of the volume of...
George Sitterley
BioFiles 2008, 3.8, 5.
Keywords: Centrifugation, Dialysis, Filtration, Purification, Sterilizations

Collagen Usage Recommendations

Collagen is one of the most abundant proteins in the connective tissues and internal organs of mammals. It provides the tensile strength of the extracellular matrix (ECM). It is classified into a num...
George Sitterley
BioFiles 2008, 3.8, 4.
Keywords: Cell culture, Morphogenesis

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

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92210 Timestrip Plus 0 °C
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