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C0551 Sigma

Monoclonal Anti-CD11b antibody produced in mouse

clone ICRF44, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-CD11b

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   human
application(s)   flow cytometry: 5 μL using 1 × 106 cells
clone   ICRF44, monoclonal
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 165-170 kDa
shipped in   wet ice
storage temp.   2-8°C
Gene Information   human ... ITGAM(3684)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human monocytes.

General description

CD11b is also known as Mac-1, CR3, MO-1 and C3bi receptor. It belongs to β 2 integrin family. The gene encodes the integrin α M chain. It is expressed on the surface of circulating monocytes, granulocytes, neutrophils, macrophages, dendritic cells and certain NK cells. It is present in subcellular granules in granulocytes and on subsets of macrophages.

Antigen Background

CD11b α-chain of the human CD11b/CD18 complex is an α/β heterodimeric glycoprotein which belongs to the β2 integrin family. It is also known as Mac-1, CR3, MO-1 and C3bi receptor. CD11b is expressed on the surface of circulating monocytes, granulocytes and certain NK cells and is also present in subsets of macrophages. It functions as a receptor for iC3b, clotting factor X, fibrinogen and ICAM-1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Specificity

Recognizes the CD11b α-chain of the human CD11b/CD18 complex. The antibody is useful for studies on cell adhesion and migration and for the detection of leukocyte adhesion deficiencies. It may be used to stain acetone-fixed cryostat sections or cell preparations. The epitope recognized by the antibody is formalin sensitive.
2nd Workshop: code no. M104
3rd Workshop: code no. 202 and 238
4th Workshop: code no. M47

Application

Monoclonal Anti-CD11b antibody produced in mouse is suitable for flow cytometry at 5μL using 1 × 106 cells.

Biochem/physiol Actions

CD11b/CD18 acts as a receptor for C3bi, clotting factor X, fibrinogen and ICAM-1. It is involved in cell-cell and cell-substrate interactions. It plays a crucial role in several immunological processes as well including leukocyte extravasation and phagocytosis. It also plays an important role in certain coagulation protease cascade and in cell migration.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Peer-Reviewed Papers
15

References

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