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C0581 Sigma

Monoclonal Anti-Calcineurin (β-Subunit) antibody produced in mouse

clone CN-B1, ascites fluid

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies for Kinase/Phosphatase Biology,
species reactivity   rat, bovine, mouse, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using neurons in human myenteric and prostatic ganglia
  indirect ELISA: suitable
  western blot: 1:3,000 using rat brain extract
clone   CN-B1, monoclonal
antibody form   ascites fluid
isotype   IgG2b
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... PPP3R1(5534), PPP3R2(5535)
rat ... Ppp3r1(29748), Ppp3r2(29749)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

bovine brain calcineurin

General description

Calcineurin (CaN, heterodimeric enzyme) is a Ca(2+)/calmodulin (CaM)-stimulated protein phosphatase 3 (PPP3) consisting of a catalytic A subunit (CnA) and a Ca(2+)-binding regulatory B subunit (CnB). The calcium-binding regulatory subunit (calcineurin B) is coded by the PPP3R1 gene located on human chromosome 2. Protein phosphatase 3, regulatory subunit B, alpha(PPP3R1) is also known as CALN, CCN1, CNA1, CALNA, PPP2B and CALNA1. The regulatory subunit (calcineurin B) is widely expressed in brain while the catalytic subunit (calcineurin A) is abundantly expressed in Hassall′s corpuscles, localized in the thymic medulla and represents the terminal stages of thymic medullary epithelium.

Specificity

In immunoblotting, the antibody recognizes an epitope located on the β-subunit of calcineurin (18 kDa, also called calcineurin B) and does not cross-react with the α-subunit.

Application

Monoclonal Anti-Calcineurin (β-Subunit) antibody produced in mouse is suitable for the following applications:
• Immunohistochemistry (formalin-fixed, paraffin-embedded sections) using neurons in human myenteric and prostatic ganglia
• Indirect ELISA
• Western blotting at a working dilution of 1:3,000 using rat brain extract

Biochem/physiol Actions

Calcineurin is a major soluble calmodulin-binding protein in the brain. It stimulates the expression of the surface molecules CD83, CD80, CD86, CD40 and HLA-DR by promoting secretion of inflammatory cytokines IL-6, TNF-α and IL-1β by human PBMC-derived dendritic cells. It plays an important role in signal transduction, activation of T cell. Calcineurin is an excellent marker enzyme for the detection of neuronal activity and synaptic plasticity after brain damage.

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