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C0678 Sigma

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse

clone VC1.1, ascites fluid, buffered aqueous solution

Synonym: Anti-CD57, Anti-N-CAM, Monoclonal Anti-HNK-1

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
species reactivity   feline, human, mouse, rat
application(s)   western blot: 1:4,000 using cerebral cortex extract
clone   VC1.1, monoclonal
antibody form   ascites fluid
form   buffered aqueous solution
isotype   IgM
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... B3GAT1(27087)
mouse ... B3gat1(76898)
rat ... B3gat1(117108)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

A homogenate of cat primary visual cortex (area 17)

Antigen Background

CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells.

Specificity

Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan.
5th Workshop: code no. NK 67

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse is suitable for western blotting with a dilution of 1:4,000 using freshly prepared extract from rat brain.

Biochem/physiol Actions

HNK1 (human natural killer-1) is referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP. It is a member of the glucuronyltransferase gene family. Human natural killer-1 (HNK1) carbohydrate epitope is expressed on various cell adhesion molecules in the nervous system and plays a role in cell-cell and cell-substrate interactions. HNK-1 epitope stabilizes GluR2 on neuronal surface membranes and regulates cell surface stability of GluR2 by modulating the interaction with N-cadherin. It is also plays a role in cell migration and synaptic plasticity. HNK-1 epitope has been associated with the risk of metastasis. It is observed to be highly expressed in melanoma cell lines.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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