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  • C0728 - Anti-m-Calpain (Domain III), Large Subunit antibody produced in rabbit

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C0728 Sigma

Anti-m-Calpain (Domain III), Large Subunit antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   mouse, rat, human
application(s)   western blot: 1:1,000
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CAPN3(825)
mouse ... Capn3(12335)
rat ... Capn3(29155)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminal of the domain-III in the large subunit of rat m-calpain (E.C. 3.4.22.17, calpain-II, protein kinase-C activating factor).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 50% glycerol, 1% BSA and 0.02% sodium azide

Specificity

By immunoblotting against the reduced protein, the antibody reacts with bands at 80 kDa and 58 kDa and a series of further cleaved active forms. It also reacts with non-reduced m-calpain. The antibody does not cross-react with other calpain family members (μ-calpain, calpain-94, ncl-2, ncl-3, etc.).

Application

Anti-m-Calpain (Domain III), Large Subunit antibody produced in rabbit is suitable for western blotting at a working dilution of 1:1,000 using an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as substrate.

Biochem/physiol Actions

The calpains are calcium-activated non-lysosomal thiolproteases. m-Calpain is called as calpain-II. It is an intracellular, Ca2+-dependent cysteine protease. m- and μ-calpain are ubiquitously expressed and are countered by the endogenous calpain inhibitor calpastatin. The calpain family members are heterodimers. Calpains are present in all mammalian tissues and are involved in a variety of processes including cell proliferation, differentiation, vesicle secretion and others.

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