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  • C0728 - Anti-m-Calpain (Domain III), Large Subunit antibody produced in rabbit

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C0728 Sigma

Anti-m-Calpain (Domain III), Large Subunit antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Intracellular Calcium Signaling, Antibodies to Calcium-Associated Proteins,
species reactivity   mouse, rat, human
application(s)   western blot: 1:1,000
clone   polyclonal
concentration   ~1 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... CAPN3(825)
mouse ... Capn3(12335)
rat ... Capn3(29155)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to the N-terminal of the domain-III in the large subunit of rat m-calpain (E.C. 3.4.22.17, calpain-II, protein kinase-C activating factor).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 50% glycerol, 1% BSA and 0.02% sodium azide

Specificity

By immunoblotting against the reduced protein, the antibody reacts with bands at 80 kDa and 58 kDa and a series of further cleaved active forms. It also reacts with non-reduced m-calpain. The antibody does not cross-react with other calpain family members (μ-calpain, calpain-94, ncl-2, ncl-3, etc.).

Application

Anti-m-Calpain (Domain III), Large Subunit antibody produced in rabbit is suitable for western blotting at a working dilution of 1:1,000 using an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as substrate.

Biochem/physiol Actions

The calpains are calcium-activated non-lysosomal thiolproteases. m-Calpain is called as calpain-II. It is an intracellular, Ca2+-dependent cysteine protease. m- and μ-calpain are ubiquitously expressed and are countered by the endogenous calpain inhibitor calpastatin. The calpain family members are heterodimers. Calpains are present in all mammalian tissues and are involved in a variety of processes including cell proliferation, differentiation, vesicle secretion and others.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Alzheimer's Disease

Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions. Hallmark pathohistological findings of AD include widespread ...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 4–8
Keywords: Alzheimer Disease, Gene expression, Genetic, Genetics, Inflammation, Metabolism, Phosphorylations

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Peer-Reviewed Papers
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