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C0730 Sigma

Anti-CD137 antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-4-1BB

Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
species reactivity   human
application(s)   capture ELISA: 0.2-0.8 μg/mL
  flow cytometry: 2.5 μg using 106 human T cells treated with PHA
  immunocytochemistry: 5-15 μg/mL
  western blot: 0.1 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   human ... TNFRSF9(3604)
biological source   goat
impurities   <0.10 EU/μg endotoxin
conjugate   unconjugated

Description

Immunogen

mouse myeloma cell line NS0-derived recombinant human 41BB/TNFRSF9/CD137 Leu24Gln186

General description

CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Application

Anti-CD137 antibody produced in goat is suitable for the following applications:
• Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
• ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
• Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
• Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
• Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS

Biochem/physiol Actions

CD137 in T lymphocytes helps to regulate activation, proliferation and apoptosis. CD137 and CD28 are co-stimulatory molecules of T cell activation. It plays an important role in regulating T-cell-dependent immune responses. The expression of CD137 correlates negatively with lymphocyte proliferation and induces cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and helps in prolongs survival.

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